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Computer simulation as a tool for the rapid optimization of the HPLC separation of a tryptic digest of human growth hormone
Summary
Type:
HPLC
Application #:
108070
Catalog#:
720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection:
UV, 220nm
Conditions:
temperature 40°C
eluent A: 0.1% trifluoroacetic acid (TFA) in water, eluent B: 0.08% TFA in acetonitrile, upper trace linear gradient from 0 to 60% B in 30min, bottom trace linear gradient from 0 to 60% B in 120min
flow rate 1ml/min
Authors:
Chloupek RC
Source:
J Chromatogr 594 (1992) 65 - 73
Details
Legend: Fig. 1. Experimental separations of the tryptic digest of rhGH used as input for computer simulation, 100µg samples injected in 100µl digestion buffer, 1. T7, 2. T14, 3. T14c, 4. T12, 5. T10a, 6. T1
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